Johns Hopkins Manual for GI Endoscopic Nurses, Third Edition

Mouen Khashab, MD; Toshunia Robinson, MD; Anthony Kalloo, MD

Chapter 50: Acid Perfusion Test (Bernstein Test) (continued)

Mouen A . Khashab, MD

The acid perfusion or Bernstein test may be used to diagnose gastroesophageal reflux disease (GERD). It is performed in the GI laboratory by alternately infusing normal saline and diluted 0.1 N HCl (hydrochloric acid) into the distal esophagus. The Bernstein test confirms sensitivity to acid in the esophagus. During the procedure, the patient is blinded to the infusion of 60 to 80 mL of 0.1 N HCl or normal saline. The infusion is introduced into a nasogastric tube (placed at 30 cm) into the esophagus at a rate of 6 to 8 mL/minute. The nurse is responsible for recording the patient’s…