- Ophthalmic Surgery, Lasers and Imaging
- May/June 2012 - Volume 43 · Issue 3: 252-256
The authors demonstrate the feasibility and advantage of spectral-domain optical coherence tomography (SD-OCT) for single-shot ocular biometric measurement during the development of the mouse eye. A high-resolution SD-OCT system was built for single-shot imaging of the whole mouse eye in vivo. The axial resolution and imaging depth of the system are 4.5 μm (in tissue) and 5.2 mm, respectively. The system is capable of acquiring a cross-sectional OCT image consisting of 2,048 depth scans in 85 ms. The imaging capability of the SD-OCT system was validated by imaging the normal ocular growth and experimental myopia model using C57BL/6J mice. The biometric dimensions of the mouse eye can be calculated directly from one snapshot of the SD-OCT image. The biometric parameters of the mouse eye including axial length, corneal thickness, anterior chamber depth, lens thickness, vitreous chamber depth, and retinal thickness were successfully measured by the SD-OCT. In the normal ocular growth group, the axial length increased significantly from 28 to 82 days of age (P < .001). The lens thickness increased and the vitreous chamber depth decreased significantly during this period (P < .001 and P = .001, respectively). In the experimental myopia group, there were significant increases in vitreous chamber depth and axial length in comparison to the control eyes (P = .040 and P < .001, respectively). SD-OCT is capable of providing single-shot direct, fast, and high-resolution measurements of the dimensions of young and adult mouse eyes. As a result, SD-OCT is a potentially powerful tool that can be easily applied to research in eye development and myopia using small animal models.
From the Optical Electronic Information and Computer Engineering College, University of Shanghai for Science and Technology, Shanghai, People’s Republic of China; the Department of Ophthalmology (MJ, MEF, XZ, SJ) and the Institute for Genetic Medicine (P-CW, MEF, TI), Keck School of Medicine, University of Southern California, Los Angeles, California; the Departments of Ophthalmology (P-CW) and Pediatric Dentistry (C-LT), Chang Gung Memorial Hospital-Kaohsiung Medical Center, and Chang Gung University College of Medicine, Taiwan.
Supported in part by National Institutes of Health grant 7R21EB008800-02 to Dr. Jiao and R01 EY09828 to Dr. Fini.
The authors have no financial or proprietary interest in the materials presented herein.
The first two authors contributed equally to this study.
Address correspondence to Shuliang Jiao, PhD, DVRC 307, University of Southern California, 1450 San Pablo Street, Los Angeles, CA 90033. E-mail: firstname.lastname@example.org
Received: May 12, 2011
Accepted: February 06, 2012
Posted Online: March 15, 2012